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Phagocytosis in MMM Cells using pHrodo™ Red E coli BioParticles® Conjugate® (Catalog # P) pHrodo™ Dyes in Flow Cytometry MMM cells were plated in glass bottomed 35mm dishes and pHrodo™ E coli BioParticles® conjugate for phagocytosis (P) were added at 50 mg/mL in HBSS (, supplemented with mM HEPES, pH adjusted to 75 with NaOH) and imaged using TRITC filtersThe following protocol is for E coli reference samples labeled with the following fluorophores Pacific Blue, Pacific Orange, BODIPYFL, Oregon Green 514, Alexa fluor 532, Alexa fluor 546, Rhodamine RedX and Texas RedX on a laser scanning confocal microscope equipped with 32anode spectral detector as in Valm et al 11Escherichia coli bacteria, immunofluorescence deconvolution micrograph The GTPase protein Rac1, which regulates cellular processes including the cell cycle and motility, is red Actin protein filaments, which make up part of the cytoskeleton, are green Frontiers A Fluorescence Based Proliferation Assay For The Identification